Methods

In our group EC-sEVs are isolated from conditioned media of primary feto-placental endothelial cells (ECs) via differential ultracentrifugation.

Endothelial cell are the cells forming the endothelium, i.e. the lining of blood vessels. They are regulators of the placental barrier and vascular tone and a site of gas and…

Hofbauer cells are macrophages in placental tissue. They are of fetal origin and act not only as immune cells, but apparently fulfill many other functions, e.g. tissue remodeling and homeostasis.

Ex vivo perfusion of the placenta was developed around 40 years ago. It is a delicate method that requires well-trained, experienced personnel. A small functional unit of the placenta, called…